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Three-Dimensional Characterization of Tethered Microspheres by Total Internal Reflection Fluorescence Microscopy

Tethered particle microscopy is a powerful tool to study the dynamics of DNA molecules and DNA-protein complexes in single-molecule experiments. We demonstrate that stroboscopic total internal reflection microscopy can be used to characterize the three-dimensional spatiotemporal motion of DNA-tether...

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Detalhes bibliográficos
Main Authors: Blumberg, Seth, Gajraj, Arivalagan, Pennington, Matthew W., Meiners, Jens-Christian
Formato: Artigo
Idioma:Inglês
Publicado em: Biophysical Society 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1366611/
https://ncbi.nlm.nih.gov/pubmed/15923224
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.105.061242
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