Picosecond-hetero-FRET microscopy to probe protein-protein interactions in live cells.

By using a novel time- and space-correlated single-photon counting detector, we show that fluorescence resonance energy transfer (FRET) between cyan fluorescent protein (CFP) and yellow fluorescent protein (YFP) fused to herpes simplex virus thymidine kinase (TK) monomers can be used to reveal homod...

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Main Authors: Tramier, Marc, Gautier, Isabelle, Piolot, Tristan, Ravalet, Sylvie, Kemnitz, Klaus, Coppey, Jacques, Durieux, Christiane, Mignotte, Vincent, Coppey-Moisan, Maïté
Formato: Artigo
Idioma:Inglês
Publicado: 2002
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Research Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC1302432/
https://ncbi.nlm.nih.gov/pubmed/12496124
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