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Picosecond-hetero-FRET microscopy to probe protein-protein interactions in live cells.

By using a novel time- and space-correlated single-photon counting detector, we show that fluorescence resonance energy transfer (FRET) between cyan fluorescent protein (CFP) and yellow fluorescent protein (YFP) fused to herpes simplex virus thymidine kinase (TK) monomers can be used to reveal homod...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Tramier, Marc, Gautier, Isabelle, Piolot, Tristan, Ravalet, Sylvie, Kemnitz, Klaus, Coppey, Jacques, Durieux, Christiane, Mignotte, Vincent, Coppey-Moisan, Maïté
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2002
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1302432/
https://ncbi.nlm.nih.gov/pubmed/12496124
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