Thermodynamics of specific and nonspecific DNA binding by two DNA-binding domains conjugated to fluorescent probes.

The complexes designed in this work combine the sequence-specific binding properties of helix-turn-helix DNA-binding motifs with intercalating cyanine dyes. Thermodynamics of the Hin recombinase and Tc3 transposase DNA-binding domains with and without the conjugated dyes were studied by fluorescence...

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Bibliographic Details
Main Authors: Thompson, M, Woodbury, N W
Format: Artigo
Language:Inglês
Published: 2001
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1301654/
https://ncbi.nlm.nih.gov/pubmed/11509389
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