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DNA-binding interactions and conformational fluctuations of Tc3 transposase DNA binding domain examined with single molecule fluorescence spectroscopy.

The fluorescent dye tetramethylrhodamine (TMR) was conjugated to a synthetic peptide containing the sequence-specific DNA binding domain of Tc3 transposase. Steady-state and single molecule fluorescence spectroscopy was used to investigate protein conformational fluctuations and the thermodynamics o...

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Detaylı Bibliyografya
Asıl Yazarlar: Daniel, Douglas C, Thompson, Martin, Woodbury, Neal W
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 2002
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC1301963/
https://ncbi.nlm.nih.gov/pubmed/11867477
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