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Characterization of the oligomeric states of insulin in self-assembly and amyloid fibril formation by mass spectrometry.

The self-assembly and aggregation of insulin molecules has been investigated by means of nanoflow electrospray mass spectrometry. Hexamers of insulin containing predominantly two, but up to four, Zn(2+) ions were observed in the gas phase when solutions at pH 4.0 were examined. At pH 3.3, in the abs...

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Autors principals: Nettleton, E J, Tito, P, Sunde, M, Bouchard, M, Dobson, C M, Robinson, C V
Format: Artigo
Idioma:Inglês
Publicat: 2000
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1301001/
https://ncbi.nlm.nih.gov/pubmed/10920035
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