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Reactions of cysteines substituted in the amphipathic N-terminal tail of a bacterial potassium channel with hydrophilic and hydrophobic maleimides

Single cysteine-substitution mutants of KcsA, a K(+) channel from Streptomyces lividans, were expressed in Escherichia coli, and inner membranes were isolated. The rate constants for the reactions of these cysteines with three maleimides of increasing hydrophobicity, 4-(N-maleimido)phenyltrimethylam...

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מידע ביבליוגרפי
Main Authors: Li, Jing, Xu, Qiang, Cortes, D. Marien, Perozo, Eduardo, Laskey, Aaron, Karlin, Arthur
פורמט: Artigo
שפה:Inglês
יצא לאור: The National Academy of Sciences 2002
נושאים:
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC129316/
https://ncbi.nlm.nih.gov/pubmed/12189213
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.192439299
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