Cloning of Escherichia coli lacZ and lacY Genes and Their Expression in Gluconobacter oxydans and Acetobacter liquefaciens

An efficient transformation protocol for Gluconobacter oxydans and Acetobacter liquefaciens strains was developed by preparation of electrocompetent cells grown on yeast extract-ethanol medium. Plasmid pBBR122 was used as broad-host-range vector to clone the Escherichia coli lacZY genes in G. oxydan...

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Bibliografiske detaljer

Udgivet i:	Appl Environ Microbiol
Principais autores:	Mostafa, Hesham E., Heller, Knut J., Geis, Arnold
Format:	Artigo
Sprog:	Inglês
Udgivet:	American Society for Microbiology (ASM) 2002
Fag:	Physiology and Biotechnology
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC127584/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11976147/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.68.5.2619-2623.2002
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