Cloning of Escherichia coli lacZ and lacY Genes and Their Expression in Gluconobacter oxydans and Acetobacter liquefaciens

An efficient transformation protocol for Gluconobacter oxydans and Acetobacter liquefaciens strains was developed by preparation of electrocompetent cells grown on yeast extract-ethanol medium. Plasmid pBBR122 was used as broad-host-range vector to clone the Escherichia coli lacZY genes in G. oxydan...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Mostafa, Hesham E., Heller, Knut J., Geis, Arnold
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2002
Assuntos:	Physiology and Biotechnology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC127584/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11976147/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.68.5.2619-2623.2002
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