Cloning of Escherichia coli lacZ and lacY Genes and Their Expression in Gluconobacter oxydans and Acetobacter liquefaciens

An efficient transformation protocol for Gluconobacter oxydans and Acetobacter liquefaciens strains was developed by preparation of electrocompetent cells grown on yeast extract-ethanol medium. Plasmid pBBR122 was used as broad-host-range vector to clone the Escherichia coli lacZY genes in G. oxydan...

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Veröffentlicht in:	Appl Environ Microbiol
Hauptverfasser:	Mostafa, Hesham E., Heller, Knut J., Geis, Arnold
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 2002
Schlagworte:	Physiology and Biotechnology
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC127584/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11976147/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.68.5.2619-2623.2002
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