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Cloning of genes coding for L-sorbose and L-sorbosone dehydrogenases from Gluconobacter oxydans and microbial production of 2-keto-L-gulonate, a precursor of L-ascorbic acid, in a recombinant G. oxydans strain.

We have purified L-sorbose dehydrogenase (SDH) and L-sorbosone dehydrogenase (SNDH) from Gluconobacter oxydans T-100 that showed an ability to convert D-sorbitol to 2-keto-L-gulonate (2-KLGA). A genomic library of Gluconobacter oxydans T-100 was screened with a probe, a 180-bp PCR product which was...

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Publicado no:Appl Environ Microbiol
Principais autores: Saito, Y, Ishii, Y, Hayashi, H, Imao, Y, Akashi, T, Yoshikawa, K, Noguchi, Y, Soeda, S, Yoshida, M, Niwa, M, Hosoda, J, Shimomura, K
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1997
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC168335/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9023923/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.63.2.454-460.1997
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