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Fluorescence polarization of green fluorescence protein

We report here the striking anisotropy of fluorescence exhibited by crystals of native green fluorescence protein (GFP). The crystals were generated by water dialysis of highly purified GFP obtained from the jellyfish Aequorea. We find that the fluorescence becomes six times brighter when the excita...

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Bibliografiske detaljer
Main Authors: Inoué, Shinya, Shimomura, Osamu, Goda, Makoto, Shribak, Mykhailo, Tran, P. T.
Format: Artigo
Sprog:Inglês
Udgivet: The National Academy of Sciences 2002
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC123638/
https://ncbi.nlm.nih.gov/pubmed/11929998
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.062065199
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