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Uncovering the hidden ground state of green fluorescent protein

The fluorescence properties of GFP are strongly influenced by the protonation states of its chromophore and nearby amino acid side chains. In the ground state, the GFP chromophore is neutral and absorbs in the near UV. Upon excitation, the chromophore is deprotonated, and the resulting anionic chrom...

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Detalhes bibliográficos
Main Authors: Kennis, John T. M., Larsen, Delmar S., van Stokkum, Ivo H. M., Vengris, Mikas, van Thor, Jasper J., van Grondelle, Rienk
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC539731/
https://ncbi.nlm.nih.gov/pubmed/15608070
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0404262102
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