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Uncovering the hidden ground state of green fluorescent protein
The fluorescence properties of GFP are strongly influenced by the protonation states of its chromophore and nearby amino acid side chains. In the ground state, the GFP chromophore is neutral and absorbs in the near UV. Upon excitation, the chromophore is deprotonated, and the resulting anionic chrom...
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| Главные авторы: | , , , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
National Academy of Sciences
2004
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC539731/ https://ncbi.nlm.nih.gov/pubmed/15608070 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0404262102 |
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