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Fluorescence polarization of green fluorescence protein

We report here the striking anisotropy of fluorescence exhibited by crystals of native green fluorescence protein (GFP). The crystals were generated by water dialysis of highly purified GFP obtained from the jellyfish Aequorea. We find that the fluorescence becomes six times brighter when the excita...

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Autori principali: Inoué, Shinya, Shimomura, Osamu, Goda, Makoto, Shribak, Mykhailo, Tran, P. T.
Natura: Artigo
Lingua:Inglês
Pubblicazione: The National Academy of Sciences 2002
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC123638/
https://ncbi.nlm.nih.gov/pubmed/11929998
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.062065199
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