Solution NMR spectroscopy of [α-(15)N]lysine-labeled rhodopsin: The single peak observed in both conventional and TROSY-type HSQC spectra is ascribed to Lys-339 in the carboxyl-terminal peptide sequence

[α-(15)N]Lysine-labeled rhodopsin, prepared by expression of a synthetic gene in HEK293 cells, was investigated both by conventional and transverse relaxation optimized spectroscopy-type heteronuclear single quantum correlation spectroscopy. Whereas rhodopsin contains 11 lysines, 8 in cytoplasmic lo...

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Detalhes bibliográficos
Main Authors: Klein-Seetharaman, J., Reeves, P. J., Loewen, M. C., Getmanova, E. V., Chung, J., Schwalbe, H., Wright, P. E., Khorana, H. G.
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences 2002
Assuntos:
Biological Sciences
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC122544/
https://ncbi.nlm.nih.gov/pubmed/11904408
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.052713999
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