Solution NMR spectroscopy of [α-(15)N]lysine-labeled rhodopsin: The single peak observed in both conventional and TROSY-type HSQC spectra is ascribed to Lys-339 in the carboxyl-terminal peptide sequence

[α-(15)N]Lysine-labeled rhodopsin, prepared by expression of a synthetic gene in HEK293 cells, was investigated both by conventional and transverse relaxation optimized spectroscopy-type heteronuclear single quantum correlation spectroscopy. Whereas rhodopsin contains 11 lysines, 8 in cytoplasmic lo...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Klein-Seetharaman, J., Reeves, P. J., Loewen, M. C., Getmanova, E. V., Chung, J., Schwalbe, H., Wright, P. E., Khorana, H. G.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 2002
Assuntos:	Biological Sciences
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC122544/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11904408/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.052713999
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