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Solution NMR spectroscopy of [α-(15)N]lysine-labeled rhodopsin: The single peak observed in both conventional and TROSY-type HSQC spectra is ascribed to Lys-339 in the carboxyl-terminal peptide sequence
[α-(15)N]Lysine-labeled rhodopsin, prepared by expression of a synthetic gene in HEK293 cells, was investigated both by conventional and transverse relaxation optimized spectroscopy-type heteronuclear single quantum correlation spectroscopy. Whereas rhodopsin contains 11 lysines, 8 in cytoplasmic lo...
সংরক্ষণ করুন:
| প্রধান লেখক: | , , , , , , , |
|---|---|
| বিন্যাস: | Artigo |
| ভাষা: | Inglês |
| প্রকাশিত: |
The National Academy of Sciences
2002
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| বিষয়গুলি: | |
| অনলাইন ব্যবহার করুন: | https://ncbi.nlm.nih.gov/pmc/articles/PMC122544/ https://ncbi.nlm.nih.gov/pubmed/11904408 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.052713999 |
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