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Biotin synthase from Escherichia coli: isolation of an enzyme-generated intermediate and stoichiometry of S-adenosylmethionine use.

A cell-free extract from Escherichia coli containing an E. coli biotin synthase that was expressed to approx. 1% of soluble cell protein by cloning the E. coli bioB gene was used to investigate the biotin synthase reaction. The pH optimum was between 8 and 8.5, and the reaction velocity was dependen...

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Main Authors: Shaw, N M, Birch, O M, Tinschert, A, Venetz, V, Dietrich, R, Savoy, L A
格式: Artigo
語言:Inglês
出版: 1998
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC1219247/
https://ncbi.nlm.nih.gov/pubmed/9494071
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