Modeling of 5′ Nuclease Real-Time Responses for Optimization of a High-Throughput Enrichment PCR Procedure for Salmonella enterica

The performance of a 5′ nuclease real-time PCR assay was studied to optimize an automated method of detection of preenriched Salmonella enterica cells in buffered peptone water (BPW). The concentrations and interactions of the PCR reagents were evaluated on the basis of two detection responses, the...

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Detalhes bibliográficos

Publicado no:	J Clin Microbiol
Principais autores:	Knutsson, Rickard, Löfström, Charlotta, Grage, Halfdan, Hoorfar, Jeffrey, Rådström, Peter
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2002
Assuntos:	Clinical Veterinary Microbiology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC120126/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11773092/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.40.1.52-60.2002
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