Modeling of 5′ Nuclease Real-Time Responses for Optimization of a High-Throughput Enrichment PCR Procedure for Salmonella enterica

The performance of a 5′ nuclease real-time PCR assay was studied to optimize an automated method of detection of preenriched Salmonella enterica cells in buffered peptone water (BPW). The concentrations and interactions of the PCR reagents were evaluated on the basis of two detection responses, the...

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Bibliografiske detaljer

Udgivet i:	J Clin Microbiol
Principais autores:	Knutsson, Rickard, Löfström, Charlotta, Grage, Halfdan, Hoorfar, Jeffrey, Rådström, Peter
Format:	Artigo
Sprog:	Inglês
Udgivet:	American Society for Microbiology (ASM) 2002
Fag:	Clinical Veterinary Microbiology
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC120126/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11773092/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.40.1.52-60.2002
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