Modeling of 5′ Nuclease Real-Time Responses for Optimization of a High-Throughput Enrichment PCR Procedure for Salmonella enterica

The performance of a 5′ nuclease real-time PCR assay was studied to optimize an automated method of detection of preenriched Salmonella enterica cells in buffered peptone water (BPW). The concentrations and interactions of the PCR reagents were evaluated on the basis of two detection responses, the...

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Pubblicato in:	J Clin Microbiol
Autori principali:	Knutsson, Rickard, Löfström, Charlotta, Grage, Halfdan, Hoorfar, Jeffrey, Rådström, Peter
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 2002
Soggetti:	Clinical Veterinary Microbiology
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC120126/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11773092/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.40.1.52-60.2002
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