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Structural Determinants of Ras-Raf Interaction Analyzed in Live Cells
The minimum structure of the Raf-1 serine/threonine kinase that recognizes active Ras was used to create a green fluorescent fusion protein (GFP) for monitoring Ras activation in live cells. In spite of its ability to bind activated Ras in vitro, the Ras binding domain (RBD) of Raf-1 (Raf-1[51-131]G...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
The American Society for Cell Biology
2002
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC117316/ https://ncbi.nlm.nih.gov/pubmed/12134072 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1091/mbc.E02-01-0019 |
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