Label-free hybridization detection of a single nucleotide mismatch by immobilization of molecular beacons on an agarose film

We developed a new technique to immobilize a set of molecular beacons on an agarose film-coated slide and found that it has the ability to identify a single nucleotide difference in label-free DNA targets. The annealing properties, specificity and hybridization dynamics of the present technique were...

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Bibliografiske detaljer

Udgivet i:	Nucleic Acids Res
Principais autores:	Wang, Hong, Li, Jiong, Liu, Heping, Liu, Quanjun, Mei, Qian, Wang, Yijin, Zhu, Jijun, He, Nongyue, Lu, Zuhong
Format:	Artigo
Sprog:	Inglês
Udgivet:	Oxford University Press 2002
Fag:	NAR Methods Online
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC117303/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12060699/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnf061
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