Label-free hybridization detection of a single nucleotide mismatch by immobilization of molecular beacons on an agarose film
We developed a new technique to immobilize a set of molecular beacons on an agarose film-coated slide and found that it has the ability to identify a single nucleotide difference in label-free DNA targets. The annealing properties, specificity and hybridization dynamics of the present technique were...
Guardado en:
| Publicado en: | Nucleic Acids Res |
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| Autores principales: | , , , , , , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Oxford University Press
2002
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC117303/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12060699/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnf061 |
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