In vitro analysis of nuclear mRNA export using molecular beacons for target detection
A detailed molecular characterization of nuclear mRNA export will require an in vitro system, allowing a biochemical reconstitution of transport. To this end, an mRNA export assay has been developed using digitonin-permeabilized HeLa cells and 2′-O-methyl oligoribonucleotide molecular beacons for ta...
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| Publicado no: | Nucleic Acids Res |
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| Autor principal: | |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2003
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC156741/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12771228/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gng063 |
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