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Single-molecule studies of repressor–DNA interactions show long-range interactions

We have performed single-molecule studies of GFP–LacI repressor proteins bound to bacteriophage λ DNA containing a 256 tandem lac operator insertion confined in nanochannels. An integrated photon molecular counting method was developed to determine the number of proteins bound to DNA. By using this...

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Detalhes bibliográficos
Main Authors: Wang, Y. M., Tegenfeldt, Jonas O., Reisner, W., Riehn, R., Guan, Xiao-Juan, Guo, Ling, Golding, Ido, Cox, Edward C., Sturm, James, Austin, Robert H.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2005
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1168954/
https://ncbi.nlm.nih.gov/pubmed/15994229
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0502917102
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