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High affinity nucleic acid aptamers for streptavidin incorporated into bi-specific capture ligands

We have isolated 2′-Fluoro-substituted RNA aptamers that bind to streptavidin (SA) with an affinity around 7 ± 1.8 nM, comparable with that of recently described peptide aptamers. Binding to SA was not prevented by prior saturation with biotin, enabling nucleic acid aptamers to form useful ternary c...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Tahiri-Alaoui, Abdessamad, Frigotto, Laura, Manville, Nick, Ibrahim, Jamal, Romby, Pascale, James, William
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 2002
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC115299/
https://ncbi.nlm.nih.gov/pubmed/12000850
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