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Genomic DNA Amplification from a Single Bacterium
Genomic DNA was amplified about 5 billion-fold from single, flow-sorted bacterial cells by the multiple displacement amplification (MDA) reaction, using φ 29 DNA polymerase. A 662-bp segment of the 16S rRNA gene could be accurately sequenced from the amplified DNA. MDA methods enable new strategies...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2005
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1151817/ https://ncbi.nlm.nih.gov/pubmed/15933038 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.6.3342-3347.2005 |
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