Genomic DNA Amplification from a Single Bacterium

Genomic DNA was amplified about 5 billion-fold from single, flow-sorted bacterial cells by the multiple displacement amplification (MDA) reaction, using φ 29 DNA polymerase. A 662-bp segment of the 16S rRNA gene could be accurately sequenced from the amplified DNA. MDA methods enable new strategies...

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Autores principales: Raghunathan, Arumugham, Ferguson, Harley R., Bornarth, Carole J., Song, Wanmin, Driscoll, Mark, Lasken, Roger S.
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology 2005
Materias:
Methods
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC1151817/
https://ncbi.nlm.nih.gov/pubmed/15933038
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.6.3342-3347.2005
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