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Use of ATP, dATP and their alpha-thio derivatives to study DNA ligase adenylation.

Bacteriophage-T4 and human type I DNA ligases were found capable of self-adenylating upon exposure to both ribo- and deoxyribo-[alpha-35S]thio-ATP. However, the joining reaction does not take place in the presence of the deoxyribotriphosphates. Enzyme adenylation is reversed in all cases by an exces...

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Hlavní autoři: Montecucco, A, Lestingi, M, Pedrali-Noy, G, Spadari, S, Ciarrocchi, G
Médium: Artigo
Jazyk:Inglês
Vydáno: 1990
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1149543/
https://ncbi.nlm.nih.gov/pubmed/2222417
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