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Use of Intron-Disrupted Polyadenylation Sites To Enhance Expression and Safety of Retroviral Vectors

Normal mRNA polyadenylation signals are composed of an AAUAAA motif and G/U box spaced 20 to 30 bp apart. If this spacing is increased further, then polyadenylation is disrupted. Previously it has been demonstrated that insertion of an intron will similarly disrupt this signal even though such intro...

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Publicado no:J Virol
Principais autores: Ismail, Said I., Rohll, Jonathan B., Kingsman, Susan M., Kingsman, Alan J., Uden, Mark
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2001
Assuntos:
Gene Therapy
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC113913/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11119589/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JVI.75.1.199-204.2001
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