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Kinetic mechanism and characterization of human beta-galactosidase precursor secreted by permanently transfected Chinese hamster ovary cells.

Chinese hamster ovary cell clones permanently transfected with the cDNA for human lysosomal beta-galactosidase secrete the enzyme precursor into the cell medium, from which it is purified to apparent homogeneity in a single step by affinity chromatography. The purified precursor is fully active, dis...

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Main Authors: Zhang, S, McCarter, J D, Okamura-Oho, Y, Yaghi, F, Hinek, A, Withers, S G, Callahan, J W
格式: Artigo
語言:Inglês
出版: 1994
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC1137483/
https://ncbi.nlm.nih.gov/pubmed/7998946
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