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Kinetic mechanism and characterization of human beta-galactosidase precursor secreted by permanently transfected Chinese hamster ovary cells.
Chinese hamster ovary cell clones permanently transfected with the cDNA for human lysosomal beta-galactosidase secrete the enzyme precursor into the cell medium, from which it is purified to apparent homogeneity in a single step by affinity chromatography. The purified precursor is fully active, dis...
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Hoofdauteurs: | , , , , , , |
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Formaat: | Artigo |
Taal: | Inglês |
Gepubliceerd in: |
1994
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Onderwerpen: | |
Online toegang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1137483/ https://ncbi.nlm.nih.gov/pubmed/7998946 |
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