Type 1 transforming growth factor beta: amplified expression and secretion of mature and precursor polypeptides in Chinese hamster ovary cells.

Recombinant type 1 transforming growth factor beta (TGF-beta) was expressed to high levels in CHO cells by using dihydrofolate reductase (dhfr) gene amplification. The expression plasmid was derived from the pSV2 vectors and contained, in tandem, the simian TGF-beta and mouse dhfr cDNAs. Transcripti...

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Main Authors: Gentry, L E, Webb, N R, Lim, G J, Brunner, A M, Ranchalis, J E, Twardzik, D R, Lioubin, M N, Marquardt, H, Purchio, A F
Format: Artigo
Jezik:Inglês
Izdano: 1987
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC367992/
https://ncbi.nlm.nih.gov/pubmed/3479680
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