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High-level expression of soluble rat hsc70 in Escherichia coli: purification and characterization of the cloned enzyme.

We have cloned the cDNA of rat hsc70 (clathrin-uncoating ATPase) into a T7 expression system and have expressed this enzyme in Escherichia coli. The recombinant clathrin-uncoating ATPase is in the cytosolic fraction of the bacterium and is soluble. It was purified to homogeneity by DEAE-cellulose an...

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Dettagli Bibliografici
Autori principali: Wang, C, Lee, M R
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1993
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1134567/
https://ncbi.nlm.nih.gov/pubmed/8363588
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