Cloning, Soluble Expression and Purification of High Yield Recombinant hGMCSF in Escherichia coli

Expression of human granulocyte macrophage colony stimulating factor (hGMCSF), a cytokine of therapeutic importance, as a thioredoxin (TRX) fusion has been investigated in Escherichia coli BL21 (DE3) codon plus cells. The expression of this protein was low when cloned under the T7 promoter without a...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Das, Krishna M.P., Banerjee, Sampali, Shekhar, Nivedita, Damodaran, Karpagavalli, Nair, Rahul, Somani, Sandeep, Raiker, Veena P., Jain, Shweta, Padmanabhan, Sriram
Format: Artigo
Sprache:Inglês
Veröffentlicht: Molecular Diversity Preservation International (MDPI) 2011
Schlagworte:
Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3111651/
https://ncbi.nlm.nih.gov/pubmed/21673940
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms12032064
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!

Ähnliche Einträge