Cloning, Soluble Expression and Purification of High Yield Recombinant hGMCSF in Escherichia coli

Expression of human granulocyte macrophage colony stimulating factor (hGMCSF), a cytokine of therapeutic importance, as a thioredoxin (TRX) fusion has been investigated in Escherichia coli BL21 (DE3) codon plus cells. The expression of this protein was low when cloned under the T7 promoter without a...

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Autori principali: Das, Krishna M.P., Banerjee, Sampali, Shekhar, Nivedita, Damodaran, Karpagavalli, Nair, Rahul, Somani, Sandeep, Raiker, Veena P., Jain, Shweta, Padmanabhan, Sriram
Natura: Artigo
Lingua:Inglês
Pubblicazione: Molecular Diversity Preservation International (MDPI) 2011
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3111651/
https://ncbi.nlm.nih.gov/pubmed/21673940
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms12032064
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