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Characterization of Soluble Enzyme II Complexes of the Escherichia coli Phosphotransferase System

Plasmid-encoded His-tagged glucose permease of Escherichia coli, the enzyme IIBC(Glc) (II(Glc)), exists in two physical forms, a membrane-integrated oligomeric form and a soluble monomeric form, which separate from each other on a gel filtration column (peaks 1 and 2, respectively). Western blot ana...

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Detalhes bibliográficos
Main Authors: Aboulwafa, Mohammad, Saier, Milton H.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC532404/
https://ncbi.nlm.nih.gov/pubmed/15576795
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.186.24.8453-8462.2004
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