Localization of spermine binding sites in 23S rRNA by photoaffinity labeling: parsing the spermine contribution to ribosomal 50S subunit functions

Polyamine binding to 23S rRNA was investigated, using a photoaffinity labeling approach. This was based on the covalent binding of a photoreactive analog of spermine, N(1)-azidobenzamidino (ABA)-spermine, to Escherichia coli ribosomes or naked 23S rRNA under mild irradiation conditions. The cross-li...

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Huvudupphovsmän: Xaplanteri, Maria A., Petropoulos, Alexandros D., Dinos, George P., Kalpaxis, Dimitrios L.
Materialtyp: Artigo
Språk:Inglês
Publicerad: Oxford University Press 2005
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Article
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC1129027/
https://ncbi.nlm.nih.gov/pubmed/15897324
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gki557
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