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The identification of spermine binding sites in 16S rRNA allows interpretation of the spermine effect on ribosomal 30S subunit functions

A photoreactive analogue of spermine, N(1)-azidobenzamidino (ABA)-spermine, was covalently attached after irradiation to Escherichia coli 30S ribosomal subunits or naked 16S rRNA. By means of RNase H digestion and primer extension, the cross-linking sites of ABA-spermine in naked 16S rRNA were chara...

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Bibliographic Details
Main Authors: Amarantos, Ioannis, Zarkadis, Ioannis K., Kalpaxis, Dimitrios L.
Format: Artigo
Language:Inglês
Published: Oxford University Press 2002
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC117059/
https://ncbi.nlm.nih.gov/pubmed/12087167
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