The identification of spermine binding sites in 16S rRNA allows interpretation of the spermine effect on ribosomal 30S subunit functions

A photoreactive analogue of spermine, N(1)-azidobenzamidino (ABA)-spermine, was covalently attached after irradiation to Escherichia coli 30S ribosomal subunits or naked 16S rRNA. By means of RNase H digestion and primer extension, the cross-linking sites of ABA-spermine in naked 16S rRNA were chara...

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Detalles Bibliográficos

Publicado en:	Nucleic Acids Res
Principais autores:	Amarantos, Ioannis, Zarkadis, Ioannis K., Kalpaxis, Dimitrios L.
Formato:	Artigo
Idioma:	Inglês
Publicado:	Oxford University Press 2002
Assuntos:	Article
Acceso en liña:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC117059/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12087167/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gkf404
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