Rescue of Newcastle Disease Virus from Cloned cDNA: Evidence that Cleavability of the Fusion Protein Is a Major Determinant for Virulence

A full-length cDNA clone of Newcastle disease virus (NDV) vaccine strain LaSota was assembled from subgenomic overlapping cDNA fragments and cloned in a transcription plasmid between the T7 RNA polymerase promoter and the autocatalytic hepatitis delta virus ribozyme. Transfection of this plasmid int...

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Detalles Bibliográficos

Publicado en:	J Virol
Principais autores:	Peeters, Ben P. H., de Leeuw, Olav S., Koch, Guus, Gielkens, Arno L. J.
Formato:	Artigo
Idioma:	Inglês
Publicado:	American Society for Microbiology (ASM) 1999
Assuntos:	Replication
Acceso en liña:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC112544/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10233962/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.73.6.5001-5009.1999
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