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Rescue of Newcastle Disease Virus from Cloned cDNA: Evidence that Cleavability of the Fusion Protein Is a Major Determinant for Virulence

A full-length cDNA clone of Newcastle disease virus (NDV) vaccine strain LaSota was assembled from subgenomic overlapping cDNA fragments and cloned in a transcription plasmid between the T7 RNA polymerase promoter and the autocatalytic hepatitis delta virus ribozyme. Transfection of this plasmid int...

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Udgivet i:J Virol
Principais autores: Peeters, Ben P. H., de Leeuw, Olav S., Koch, Guus, Gielkens, Arno L. J.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1999
Fag:
Replication
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC112544/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10233962/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.73.6.5001-5009.1999
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