Rescue of Newcastle Disease Virus from Cloned cDNA: Evidence that Cleavability of the Fusion Protein Is a Major Determinant for Virulence

A full-length cDNA clone of Newcastle disease virus (NDV) vaccine strain LaSota was assembled from subgenomic overlapping cDNA fragments and cloned in a transcription plasmid between the T7 RNA polymerase promoter and the autocatalytic hepatitis delta virus ribozyme. Transfection of this plasmid int...

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Bibliographic Details
Main Authors: Peeters, Ben P. H., de Leeuw, Olav S., Koch, Guus, Gielkens, Arno L. J.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 1999
Subjects:
Replication
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC112544/
https://ncbi.nlm.nih.gov/pubmed/10233962
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