Solid phase DNA amplification: characterisation of primer attachment and amplification mechanisms

Different chemical methods used to attach oligonucleotides by their 5′-end on a glass surface were tested in the framework of solid phase PCR where surface-bound instead of freely-diffusing primers are used to amplify DNA. Each method was first evaluated for its capacity to provide a high surface co...

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Bibliografiske detaljer
Main Authors: Adessi, Céline, Matton, Gilles, Ayala, Guidon, Turcatti, Gerardo, Mermod, Jean-Jacques, Mayer, Pascal, Kawashima, Eric
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2000
Fag:
NAR Methods Online
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC110803/
https://ncbi.nlm.nih.gov/pubmed/11024189
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