Solid phase DNA amplification: characterisation of primer attachment and amplification mechanisms

Different chemical methods used to attach oligonucleotides by their 5′-end on a glass surface were tested in the framework of solid phase PCR where surface-bound instead of freely-diffusing primers are used to amplify DNA. Each method was first evaluated for its capacity to provide a high surface co...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Adessi, Céline, Matton, Gilles, Ayala, Guidon, Turcatti, Gerardo, Mermod, Jean-Jacques, Mayer, Pascal, Kawashima, Eric
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 2000
Assuntos:	NAR Methods Online
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC110803/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11024189/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.20.e87
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