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Expansion of the (CTG)(n) repeat in the 5′-UTR of a reporter gene impedes translation

Effects of d(CAG)(n)·d(CTG)(n) repeats on expression of a reporter gene in human cell culture were studied using transient transfection, RNase protection and coupled transcription/translation assays. Cloning these repeats into the reporter 3′-UTR did not affect gene functioning. In contrast, placing...

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Detalhes bibliográficos
Main Authors: Raca, Gordana, Siyanova, Elena Yu., McMurray, Cynthia T., Mirkin, Sergei M.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2000
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC110791/
https://ncbi.nlm.nih.gov/pubmed/11024174
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