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Eukaryotic ribonucleases HI and HII generate characteristic hydrolytic patterns on DNA–RNA hybrids: further evidence that mitochondrial RNase H is an RNase HII
RNase H activities from HeLa cells (either of cytoplasmic or mitochondrial origin), and from mitochondria of beef heart and Xenopus ovaries, have been tested with RNA–DNA substrates of defined length (20 bp) and sequence. Substrates were either blunt-ended, or presented DNA or RNA overhangs. The hyd...
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| Main Authors: | , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
Oxford University Press
2000
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC110728/ https://ncbi.nlm.nih.gov/pubmed/10982891 |
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