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Eukaryotic ribonucleases HI and HII generate characteristic hydrolytic patterns on DNA–RNA hybrids: further evidence that mitochondrial RNase H is an RNase HII

RNase H activities from HeLa cells (either of cytoplasmic or mitochondrial origin), and from mitochondria of beef heart and Xenopus ovaries, have been tested with RNA–DNA substrates of defined length (20 bp) and sequence. Substrates were either blunt-ended, or presented DNA or RNA overhangs. The hyd...

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Bibliographische Detailangaben
Hauptverfasser: Pileur, Frédéric, Toulme, Jean-Jacques, Cazenave, Christian
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 2000
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC110728/
https://ncbi.nlm.nih.gov/pubmed/10982891
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