Insertion of disease-causing mutations in BACs by homologous recombination in Escherichia coli
We have used GET Recombination, an inducible homologous recombination system for Escherichia coli, to insert one of the most common thalassaemia mutations into the intact β-globin locus in a second generation BAC vector. We first inserted a PCR fragment carrying the tetracycline resistance gene (Tet...
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| Publicado no: | Nucleic Acids Res |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2000
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC110716/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10954612/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.17.e79 |
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