Insertion of disease-causing mutations in BACs by homologous recombination in Escherichia coli

We have used GET Recombination, an inducible homologous recombination system for Escherichia coli, to insert one of the most common thalassaemia mutations into the intact β-globin locus in a second generation BAC vector. We first inserted a PCR fragment carrying the tetracycline resistance gene (Tet...

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Dades bibliogràfiques

Publicat a:	Nucleic Acids Res
Autors principals:	Nefedov, Mikhail, Williamson, Robert, Ioannou, Panayiotis A.
Format:	Artigo
Idioma:	Inglês
Publicat:	Oxford University Press 2000
Matèries:	NAR Methods Online
Accés en línia:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC110716/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10954612/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.17.e79
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